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Jest

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Validate output by making calculations by hand


Example Calculations

*.sharedJest

Estimating the fraction of sequences that belong to shared OTUs.

Just as the Chao1 richness estimator is a function of the number of OTUs observed once or twice in a sample (6), the estimators of the fraction of sequences in shared OTUs is a function of the number of shared OTUs that are observed at least once or twice in the community being analyzed (8, 7). <math> S_{Jest}</math> is essentially a form of the equation for <math>{J_{abund}} </math> that is not corrected for the presence of unsampled OTUs.


<math>S_{Jest} = \frac{S_{A,B Chao1}}{S1_{chao} + S2_{chao} - S_{A,B Chao1}}</math>,


where,

<math>S_{A,B Chao1}</math> = estimated number of OTUs shared between A and B using the SharedChao1 estimator. S1chao = number of OTUs estimated in A using the Chao estimator. S2chao = number of OTUs estimated in B using the Chao estimator.


For this example the value was 0.88.